Figure 3.

Representative metabolite concentration maps from the central slice derived from a series of dynamic 3D ²H CSI images summed over the first 30 minutes of signal acquisition following [2,3-²H₂]fumarate injection into A11 (B, C, E, and F), S2 (H, I, K, and L), and U87 (N, O, Q, and P) tumor-bearing mice. The color code represents concentration (in mmol/L) derived from the ratios of the peak intensities in the malate and fumarate maps to peak intensities in an initial HDO map and corrected for the number of ²H labels per molecule and for signal saturation. The locations of the tumors are indicated by dotted white lines determined from an underlying T₂-weighted ¹H image acquired concurrently using a ¹H volume coil. The corresponding T₂-weighted ¹H images were acquired separately at 9.4 T. The tumor locations are outlined with dotted black lines. T₂-weighted ¹H images (A, G, and M) and the fumarate (B, H, and N) and malate (C, I, and O) concentration maps pretreatment and the corresponding T₂-weighted ¹H images (D, J, and P) and the fumarate (E, K, and Q) and malate (F, L, and R) concentration maps 48 hours following the last chemoradiotherapy treatment.