Figure 2.

Vhl^ΔOB mice do not lose trabecular bone volume during osteomyelitis. (A) Trabecular bone volume per total volume (BV/TV) in Hif1a^ΔOB mice and Hif1a^fl/fl littermate control mice (n=8-9) was measured in the distal metaphysis of infected and contralateral femurs using microCT. (B) BV/TV was measured in the infected and contralateral distal metaphyses of femurs in Vhl^ΔOB mice and Vhl^fl/fl littermate control mice (n=7-10). Squares represent males, and circles represent females; *p < 0 .05, **p < 0.01, and ns denotes not significant (p > 0.05) as determined by two‐way analysis of variance (ANOVA) (A, B). Comparison of bracketed genotypes refers to two-way ANOVA comparison of genotypes as a whole without multiple comparisons by laterality of femur, denoted with ****p < 0.0001. (C) The data from Vhl^fl/fl littermate control mice (n=10) analyzed in (B) are shown in an enlarged graph with lines denoting paired contralateral and infected femurs. ***p < 0.001, as determined by paired, two-tailed Student’s t-test. (D) Volume of cortical bone loss in the infected femur in Hif1a^ΔOB or Hif1a^fl/fl littermate control mice (n=8) was determined using microCT at post-infection day 14. Squares represent males, and circles represent females. (E) Cortical bone loss at post-infection day 14 in the infected femurs of n=5-7 male Vhl^ΔOB or Vhl^fl/fl littermate control mice was determined using microCT and is expressed as a normalized ratio relative to equivalent analysis performed on the contralateral femur. ns denotes not significant (p > 0.05) as determined by unpaired, two-tailed Student’s t-test (D, E). Error bars represent mean ± SEM.