Table 1.
Primer sequences used for RT-PCR.
| Gene | Sequences | PrimerBank ID | |
|---|---|---|---|
| Actb (β-actin) | Forward | 5’-GGCTGTATTCCCCTCCATCG-3’ | 6671509a1 |
| Reverse | 5’-CCAGTTGGTAACAATGCCATGT-3’ | ||
| Tnfsf11 (RANKL) | Forward | 5’-CAGCATCGCTCTGTTCCTGTA-3’ | 6755833a1 |
| Reverse | 5’-CTGCGTTTTCATGGAGTCTCA-3’ | ||
| Tnfrsf11b (OPG) | Forward | 5’-ACCCAGAAACTGGTCATCAGC-3’ | 31543882a1 |
| Reverse | 5’-CTGCAATACACACACTCATCACT-3’ | ||
| Vhl (VHL) | Forward | 5’-ACATCGTCAGGTCACTCTATGA-3’ | 118130344c3 |
| Reverse | 5’-CTCTTGGCTCAGTCGCTGTAT-3’ | ||
| Sp7 (Osx) | Forward | 5’-ATGGCGTCCTCTCTGCTTG-3’ | 18485518a1 |
| Reverse | 5’-TGAAAGGTCAGCGTATGGCTT-3’ |
For each gene (protein in parentheses) tested by RT-PCR, the forward and reverse primer sequences are shown along with the Harvard PrimerBank ID for each primer pair. PrimerBank (available at: https://pga.mgh.harvard.edu/primerbank/index.html) was used as the reference for each primer set.