Fig. 3.
miR-339-5p directly targets the oprm1 3’UTR. (A) pmiR-RB-REPORTTM Double luciferase reporter vector. (B) The predicted sequences for target site of r-oprm1-WT and r-oprm1-MUT (indicated by red letters). (C) Relative luciferase activity. After the transfection of rno-mir-339-5p, the reported fluorescence expression of r-oprm1-MUT increased significantly compared with that of r-oprm1-WT (*P < 0.050). This significant difference does not exist in the NC (**P > 0.050). The error bars indicate mean ± standard deviation. 3’UTR: 3’untranslated region, NC: negative control.