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. 2022 Oct 1;35(4):423–432. doi: 10.3344/kjp.2022.35.4.423

Table 2.

Informations of pmiR-RB-REPORTTM Double luciferase assay reporter gene vector

Name of sequences r-Oprm1(NM_001304733.1 complete sequence)-WT, r-Oprm1(NM_001304733.1 complete sequence)-MUT
Clone length 902 bp (NM_001304733.1 complete sequence)
5′cleavage site XhoI
3′cleavage site NotI
Name of vector pmiR-RB-ReportTM
Size of vector’ skeleton 6.3 kb
Reporter gene Renilla luciferase
Reference gene Firefly luciferase
Host bacteria Escherichia coli DH5α