Table 2.
Name of sequences | r-Oprm1(NM_001304733.1 complete sequence)-WT, r-Oprm1(NM_001304733.1 complete sequence)-MUT |
Clone length | 902 bp (NM_001304733.1 complete sequence) |
5′cleavage site | XhoI |
3′cleavage site | NotI |
Name of vector | pmiR-RB-ReportTM |
Size of vector’ skeleton | 6.3 kb |
Reporter gene | Renilla luciferase |
Reference gene | Firefly luciferase |
Host bacteria | Escherichia coli DH5α |