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. 2022 Sep 20;9:977761. doi: 10.3389/fvets.2022.977761

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Copyright © 2022 Chestley, Sroga, Nebroski, Hole, Ularamu, Lung and Nfon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Repeatability analysis for the FMDV SAT1, SAT2 and SAT3 assays. FMDV genomic RNA was extracted on three separate days from prototypic FMDV cell culture isolates SAT1/KEN/4/1998, SAT1/BOT/12/2006, SAT2/SAU/1/2000, SAT2/EGY/2/2012, SAT3/SAR/1/2006 and SAT3/ZAM/1/2017 as well as a PBS extraction control (Extn Con). Extracted RNA from each replicate was also analyzed using the SAT1 (A), SAT2 (B), and SAT3 (C) srRT-PCR assays on three separate days. The FMDV 3D pan-serotype rRT-PCR assay (FMDV) was run in parallel with each replicate to ensure the presence of the FMDV template (D). Replicate Cq values are plotted with a black line representing the mean and error bars with the corresponding FMDV serotype color (SAT1 = yellow, SAT2 = purple, SAT3 = orange and FMDV 3D pan-serotype = green).