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. 2022 Sep 20;9:977761. doi: 10.3389/fvets.2022.977761

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Copyright © 2022 Chestley, Sroga, Nebroski, Hole, Ularamu, Lung and Nfon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analytical sensitivity limit of detection (LoD) analysis for the FMDV SAT1, SAT3 and SAT2 topotype VII rRT-PCR assays. FMDV genomic RNA was extracted from duplicate 10-fold serial dilutions (10⁰ – 10⁻⁷ or 10⁻⁹) of the FMDV cell culture isolates SAT1/KEN/4/1998 (A), SAT1/KEN/121/2009 (B), SAT2/ SAU/1/2000 (C), SAT2/SEN/27/2009 (D), SAT3/ZIM/4/1981 (E), and SAT3/SAR/1/2006 (F) and were tested using the homologous serotype SAT-specific rRT-PCR assay as well as the FMDV pan-serotype 3D rRT-PCR assay. Mean Cq values are plotted with standard deviations.