Table 2.

FMDV SAT1, SAT2 and SAT3 serotype-specific rRT-PCR assay primers and probes.

FMDV serotype reagent	FMDV SAT specific oligo name	Sequence (5′ → 3′)
SAT1 forward	SAT1_3437_F	AGGCANCACACTGAYGTG
SAT1 reverse	SAT1_3502_R	GCAGRTCCAGTGTCAGTYT
SAT1 probe	SAT1_3474_P	FAM-CCTYGACCGGTTCACHCTDGT-BHQ1
SAT2 forward	SAT2_3765_F	YGTCTACAAYGGYGAGT
SAT2 reverse	SAT2_3934_R	CCKCTTCATCCKGTAGTARA
SAT2 probe	SAT2_3867_P	FAM-CGDACCCGAAGTTGAAGGTBGRCG-BHQ1
SAT3 forward	SAT3_3736_F	GYGTTGAGAMTGAAACCAC
SAT3 reverse	SAT3_ 3834_R	CWGCHCTCTTCATCCGGTA
SAT3 probe	SAT3_probe1.2	FAM-AVAGWCGCCCGAAGTTGAATGTYGTGGG-BHQ1

Characters in sequences represent degenerate bases: Y (C, T), B (C, T, G), H (C, A, T), K (G, T), M (A, C), D (A, G, T), R (A, G), W (A, T), and N (A, C, T, G).