Table 3.
Detection of FMDV A, O and Asia1 cell culture isolates with the serotype-specific SAT1, SAT3 and SAT2 topotype VII rRT-PCR assays.
| Serotype | Strain | SAT1 Cq | SAT2 Cq | SAT3 Cq | FMDV 3D Cq | Xeno Cq |
|---|---|---|---|---|---|---|
| O | OUKG | No Cq | No Cq | No Cq | 13.29 | 32.30 |
| O | O1 BFS | No Cq | No Cq | No Cq | 13.54 | 33.31 |
| O | O1 Manisa | No Cq | No Cq | No Cq | 20.14 | 33.63 |
| O | O1 Campos | No Cq | No Cq | No Cq | 13.54 | 33.57 |
| O | O/TAN/2009 | No Cq | No Cq | No Cq | 12.63 | 33.31 |
| O | O/CAR/2005 | No Cq | No Cq | No Cq | 13.88 | 33.29 |
| O | O/VIT/2012 | No Cq | No Cq | No Cq | 12.31 | 33.24 |
| O | O/LIB/2012 | No Cq | No Cq | No Cq | 10.75 | 32.88 |
| O | O/KEN/2009 | No Cq | No Cq | No Cq | 13.30 | 33.12 |
| O | O/NIG/2017 | No Cq | No Cq | No Cq | 14.78 | 33.97 |
| A | A/22 | No Cq | No Cq | No Cq | 10.67 | 33.17 |
| A | A/MAY/1997 | No Cq | No Cq | No Cq | 11.35 | 32.90 |
| A | A/COL/1985 | No Cq | No Cq | No Cq | 13.52 | 33.29 |
| A | A/IRN/1/1996 | No Cq | No Cq | No Cq | 16.69 | 32.81 |
| A | A/IRN/2005 | No Cq | No Cq | No Cq | 16.04 | 33.77 |
| A | GHA/4/1996 | No Cq | No Cq | No Cq | 11.67 | 31.41 |
| A | BKF/4/1994 | No Cq | No Cq | No Cq | 10.62 | 31.87 |
| A | ERI/2/1998 | No Cq | No Cq | No Cq | 12.50 | 31.65 |
| A | ETH/6/2000 | No Cq | No Cq | No Cq | 11.54 | 31.98 |
| A | NIG/38/2009 | No Cq | No Cq | No Cq | 13.81 | 31.41 |
| A | ETH/12/2009 | No Cq | No Cq | No Cq | 13.64 | 32.03 |
| A | EGY/3/2009 | No Cq | No Cq | No Cq | 17.85 | 32.00 |
| A | SUD/1/2006 | No Cq | No Cq | No Cq | 12.03 | 31.93 |
| A | CAR/10/2013 | No Cq | No Cq | No Cq | 12.67 | 31.56 |
| A | NIG/A/6/2019 | No Cq | No Cq | No Cq | 12.91 | 33.15 |
| A | NIG/A/7/2019 | No Cq | No Cq | No Cq | 10.58 | 31.85 |
| A | NIG/A/12/2020 | No Cq | No Cq | No Cq | 11.87 | 31.43 |
| A | NIG/A/1/2019 | No Cq | No Cq | No Cq | 14.78 | 31.74 |
| Asia1 | Asia1/Shamir/2001 | No Cq | No Cq | No Cq | 13.99 | 32.87 |
| Asia1 | Asia1/PAK/1994 | No Cq | No Cq | No Cq | 12.43 | 33.52 |
Samples are considered positive when the quantification cycle (Cq) is ≤ 35.99 for SAT1, SAT2, SAT3, FMDV pan-serotype 3D and Xeno control assays.
The colours used to indicate each of the Foot-and-Mouth Disease virus serotypes. Serotype O is blue, serotype A is red, serotype Asia1 is grey, serotype SAT1 is yellow, serotype SAT2 is purple and serotype SAT3 is orange. When the Cq value cells are highlighted with either yellow, purple or orange, it means that a Cq value was produced for this particular viral isolate or sample. If the yellow, purple or orange is darker and the Cq value is <35.99 then the sample was positive when evaluated by the corresponding assay. If the filled cell is the lighter version of the colour, it indicates that although a Cq value was produced, it is >35.99 and the sample is considered negative.