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. 2022 Sep 20;15:973646. doi: 10.3389/fnmol.2022.973646

FIGURE 2.

FIGURE 2

Confocal microscopy, SR-SIM, and Cx26 and Cx30 antibody labeling of the organ of Corti and lateral wall. (A) Cx26 and Cx30 are expressed in the spiral limbus fibrocytes. There is no expression in Reissner’s membrane (RM). (B) The OC expresses both Cx26 and Cx30, but in Figure B, only Cx30 is shown. IHC: inner hair cell. OHCs: outer hair cells. PC: pillar cell. HC, Hensen cell; DC, Deiters cell. TCL: tympanic covering layer. (C) Cx26 and Cx30 are both jointly and separately expressed in the lateral wall. Cx30 dominates except at the basal cell area of the stria vascularis. There is no expression in the marginal cells or in cells laterally margining the bone (type III fibrocytes). (D) SR-SIM using spot detection mode (Imaris 9.5.1) demonstrates separate and closely associated protein labeling with geometric arrangements in the lateral cochlear wall (D’ and D”). Cx30, but not Cx26, is expressed in the intermediate cells. The cochlear turn is not given.