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. 2022 Sep 20;15:973646. doi: 10.3389/fnmol.2022.973646

FIGURE 3.

FIGURE 3

Light microscopy and confocal microscopy of the lateral wall of the upper-middle cochlear turn (frequency location 250–500Hz) showing marginal, intermediate, and basal cells of the stria vascularis. (A) The marginal cells are believed to be true epithelial cells, but there is no continuous basal lamina. Type I fibrocytes (I) of the spiral ligament lie close to the basal cells. The spiral prominence epithelium is flatter and is related to the type II fibrocytes. The outer sulcus (OS) epithelium, and Claudius and Hensen cells have a light cytoplasm and are fairly organelle-free. Root cells (RCs) project into the subepithelial space. Type III fibrocytes are located near the bone, and type IV faces the scala tympani. (B) RNAscope shows the distribution of KCNJ10 and GJB2 gene transcripts in intermediate and basal cells. (C) GJB2 transcripts are located in the basal cells, but not in the marginal cells. (D) ATP1A1 gene transcripts are seen in the marginal cells, while the basal cells contain ATP1A2.