TABLE 1.
Oligonucleotides used in this work
| Oligonucleotide | Sequence (5′→3′)a | Complementary sequence | Plasmid(s) constructed |
|---|---|---|---|
| ARA1 | −39TAAGGGTAACTATTGCCG−22 | pSN32 | pLM53, pLM55, pLM76 |
| ARA7 | +178CCTCTTCGCTATTACGCC+161 | pSN32 | pLM53, pLM55, pLM76 |
| ARA23 | +92CCCGGATATCGAGTAAAGCGTTTTCATTTAAACC+59 | araE | pLS12, pLS14 |
| ARA24 | +47GTACTTATTTTAAAATTTCG+26 | araE | pLM38, pLM39 |
| ARA25 | +8AGTAAGTACTAATATAGAATAATCTTGTTGC−23 | araE | pLS2, pLS3, pLS9 |
| ARA26 | +17CCAAAATGAATTCGGATCCATTTCAG−9 | araR | pLM38, pLS2, pLS9, pLS12 |
| ARA27 | +67GAACTGGATCCTTCTTTGAATTCCGCGTATTTTGG+34 | araR | pLM39, pLS3, pLS14 |
| ARA28 | +87CCTATTGAATTCAAAAGCCGG−67 | araA | pLM33, pLM34, pLM35, pLM48, pLM49 |
| ARA29 | +10TTGTAGGATCCATTTTATTATAGTAATTGAC−21 | araA | pLM35 |
| ARA30 | +47CGTACTAAAGATCTAAAATAAACC+24 | araA | pLM34 |
| ARA31 | +75AATAAAACGGATCCAAATAC+56 | araA | pLM33 |
| ARA32 | +124GAATTCAGGATCCTTTGTCTGAAGC+100 | araA | pLM48, pLM49 |
| ARA33 | +18TTTATCGATTTATTTTCTATC+38 | araA | pLM48, pLM49 |
| ARA34 | +43CTAATGATCGATAATAAAC+24 | araA | pLM49 |
| ARA35 | +37ATAGAAATCGATCCTATAAATAAATATTTGTACG+4 | araA | pLM48 |
| ARA50 | +16GGAGGAATGCATATGTTACC+35 | araR | pLS16 |
| ARA51 | +1128ACATTGCTTAAGCTTTTCATTCAGTTTTCGTGCGG+1094 | araR | pLS16 |
| ARA65 | +15TTATTTAAAGCTTTATT+32ATCG | araA | pLM55 |
| ARA66 | +34GAAAATAAA+26AGCTT+31AATAAACC+24 | araA | pLM55 |
| ARA67 | +13ATTTA+17AAGCTTCGA+26TTTATTTTCTATC+38 | araA | pLM53 |
| ARA68 | +39TGATAGAAGCTTAA+26TCGAT+25CC+24 | araA | pLM53 |
| ARA72 | +35AGTGTATCAACAAGCTGG+17 | pSN32 | − |
| ARA77 | +24GGTTTATTTTCTATCATTAGTACTTATCTTTTGTATTTG+62 | araA | pLM78 |
| ARA78 | +62CAAATACAAAAGATAAGTACTAATGATAGAAAATAAACC+24 | araA | pLM78 |
| ARA82 | −139TAATTTACCGAAACTTGCGG−120 | veg | pLM87 |
| ARA83 | +75CAGCCGTTAACCTAAATTCCC+55 | veg | pLM87 |
a
The number within the primers refers to the position of the sequence in the araABDLMNPQ-abfA operon, araE, araR, and veg relative to the transcription start point of each gene or in pSN32 relative to the EcoRI site (+1) in the multiple cloning site. Restriction sites used are underlined in the oligonucleotide sequences: BamHI (GGATCC), BglII (AGATCT), ClaI (ATCGAT), DraI (TTTAAA); EcoRI (GAATTC), EcoRV (GATATC), HincII (GTTAAC), Hind (AAGCTT), NdeI (CATATG), and ScaI (AGTACT).