Figure 2.

RNA-seq expression patters between different elevation belts

Summary of the RNA-seq expression patterns by volcano plots obtained from DESeq2 analysis (x-axis represents the log2 of the fold change between the gene expression of two groups; the y-axis represents the negative decade logarithm of the adjusted p-value) for the three different comparisons between B. lucorum samples from the different elevation groups: highland vs lowland (A); highland vs mid-elevation (B); mid-elevation vs lowland (C). Red and blue points mark the genes with significantly increased or decreased expression respectively for the different group comparisons regarding always the first mentioned elevation group as up-regulated (reddish colors) and down-regulated (bluish colors). Most significant expressed genes were labeled with their name. Selected genes known to be involved in elevational adaption from other studies are highlighted in ochre (genes coding for cold shock protein), green (genes coding for heat shock protein) and purple (genes coding for octopamine protein), which all lay in the group of not significant regulated genes. Dashed lines represent applied significance thresholds.