Figure 3.

Gene set enrichment analysis (GSEA) between the different elevation belts

Enrichment map representation of the ranked GSEA results obtained for two-way comparative analysis between B. lucorum samples from each elevation gradient group: highland vs lowland (A); highland vs mid-elevation (B); mid-elevation vs lowland (C). Enrichment maps from GSEA showing gene sets in an interaction network with nodes of transcripts received by RNA-seq with moderately conservative statistical significance (p < 0.005, FDR < 0.05, and overlap coefficient = 0.2). Clustered sub networks of nodes (depicted as dotted circles) reflect generic functional networks. Nodes represent enriched gene sets, where node size corresponds to the number of genes and color intensity corresponds to normalized enrichment score. Edges represent overlap between gene sets with line thickness correlating to the degree of overlap. Pathways were manually grouped into different categories based on their KEGG assignment.