Fig. 2.

Immunocytochemistry of TMEM163 heterologously co-expressed with ZNT proteins. Representative confocal images of cells co-transfected with TMEM163-DDK and: A) ZNT1-HA, B) ZNT2-HA, C) ZNT3-HA, and D) ZNT4-HA. The DDK peptide was detected by anti-DDK mAb and visualized with anti-mouse secondary antibody conjugated with Alexa-488 (green). The HA peptide was detected by anti-HA pAb and visualized with anti-rabbit secondary antibody conjugated with Alexa-568 (red). DAPI stains the nuclei blue. Arrow indicates co-localization of TMEM163 and ZNT protein heterodimers within their respective cellular sites. Arrowhead indicates non-overlapping subcellular localization of protein homodimers. Protein co-localization was semi-quantitatively determined using the pixel-based and object-based analyses pipeline of the Cell Profiler software. Pearson correlation coefficient (PCC) shows the degree of overlapping fluorescence between the two channels. The fluorescent objects (in percent) provide the number of overlapping objects between the two channels. Scale bar: 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)