FIGURE 2.

Increased activation of JNK pathway in murine intestinal tumors by Brg1 suppression. (A) Genetic strategy for Brg1 ablation in intestinal tumor cells following tamoxifen (4‐OHT) induction in Hnf1b ^CreERT2/+; Apc ^Min/+; Brg1 ^flox/flox (HAB) mice. (B) Experimental schedule for establishment of HAB spheroids and analysis. (C) Quantitative real‐time PCR analysis of c‐Jun expression in HAB spheroids treated with MeOH or 4‐OHT (p = 0.0080; n = 4). (D) Immunohistochemistry for p‐c‐JUN in HAB spheroids treated with MeOH or 4‐OHT. Scale bars = 20 μm. (E) Genetic strategy for Dclk1+ intestinal tumor cells specific Brg1 deletion following tamoxifen (Tam) induction in Dclk1 ^{CreERT2‐IRES‐EGFP/+}; Apc ^Min/+; Brg1 ^flox/flox (DAB) mice. (F) Immunohistochemistry for p‐c‐JUN in intestinal tumors of Dclk1 ^{CreERT2‐IRES‐EGFP/+}; Apc ^Min/+; Brg1 ^flox/wt or Dclk1 ^{CreERT2‐IRES‐EGFP/+}; Apc ^Min/+; Brg1 ^wt/wt (DA) and DAB mice 5 days after the last Tam injection. Scale bars = 100 μm and 20 μm. (G) Immunohistochemistry for Brg1 and p‐c‐Jun in serial sections of intestinal tumors in DA and DAB mice at day 5 after the last Tam injection. Scale bars = 20 μm. Analyzed by Student' s t‐test. *p < 0.05, **p < 0.01, ***p < 0.001