FIGURE 1.

Genomic alterations and GPC expression in osteosarcoma. (A) Osteosarcoma‐related gene alterations were detected in 24 osteosarcomas using cancer‐targeted next‐generation sequencing. Only one patient (OS‐8) was found to have a GPC3 mutation. (B) RT‐PCR demonstration of GPC3 transcription in OSs. β‐Actin was used as a quantitative control, and OS‐8 was used as a GPC3‐positive control. (C) Sanger sequencing was performed on the GPC3 RT‐PCR product of mutant GPC3 (OS‐8) and OS‐22 as the representative GPC3 wild‐type