TABLE 2.

Potential off‐target sequences in the founder Brca1 ^L63X/+ rat

Guide RNA a	Location b	Reference sequence (5′→3′) c	Primers a	Result
G1	Target (Brca1)	GTTATCTCATTCTTACACAAAGG	N/A	N/A
	Chr 6: +73,719,173	GGAATCTTATTCTTACACAATGG	F3/R3	Intact
	Chr 13: −20,706,840	CTGAACTCATTCTTACACAAAAG	F4/R4	Intact
	Chr 14: +11,065,816	CTTCTCCCATTCTTACACAAAAG	F5/R5	Intact
	Chr 14: +11,066,038	CTTCTCCCATTCTTACACAAAAG	F5/R5	Absent d
	Chr 15: −77,797,047	TTTATGTTATTCTTACACAAAGG	F6/R6	Intact
	Chr 2: −229,084,162	TTTATCTAATTATTACACAATAG	F7/R7	Intact
	Chr 1: −280,828,323	AGTGACTCATTCTTACACAAAAG	F8/R8	Intact
G2	Target (Brca1)	ATTCTTACACAAAGGACACTGGG	N/A	N/A
	Chr 7: +111,332,798	TTCCTTACCCAAAGGACACTTAG	F9/R9	Intact e
	Chr 1: −157,626,260	TGTTTTAAACAAAGGACACTAAG	F10/R10	Intact
	Chr 20: +10,695,088	GTTTATACAGAAAGGACACTAAG	F11/R11	Intact

^a Guide RNA and primer sequences are shown in Table 1.

^b Location of the 5′‐end in the Rnor 6.0 assembly. Plus/minus signs indicate sense/antisense strands.

^c Sequence for the BN strain. Underlined bases indicate mismatches relative to the guide RNA sequence.

^d A 222 bp sequence (chromosome [Chr] 14: 11,065,964–11,066,185), present in the reference sequence of the BN strain, was absent in the founder as well as in three untreated Jcl:SD rats.

^e Single nucleotide polymorphism g.111332800C>T was present in the founder as well as in three untreated Jcl:SD rats. F, forward; N/A, not applicable; R, reverse.

The Significance of Bold values indicates guide RNA sequences.