FIGURE 5.

RNA sequencing (RNA‐Seq) of patient‐specific induced pluripotent stem cell‐derived cardiomyocytes (pt‐iPSC‐CMs) and quantitative real‐time RT‐PCR (qRT‐PCR) analysis of genes associated with cardiotoxicity. (A) Schematic illustrating the experimental workflow. RNA‐Seq was undertaken on iPSC‐CMs that were treated for 7 days with or without 1 μM trastuzumab (Tmab). (B–F) Comparisons of various gene expression levels in untreated and Tmab‐treated pt‐iPSC‐CMs using qRT‐PCR analysis (false discovery rate < 0.05 and fold change > 3) showed distinct patterns of gene expression between the severely cardiotoxic patient group (SP; n = 3) versus noncardiotoxic patient group (NP; n = 3) and Tmab‐treated versus untreated. Relative gene expressions of KLK5, KLK8, IL1B, PTGS1, and TNFRSF8 in pt‐iPSC‐CMs are shown. All data are expressed as mean ± SEM