FIG. 5.

Expression of the B. cepacia RR10 alkB gene in cells growing at the expense of alkanes of different chain lengths. B. cepacia RR10 was grown in minimal salts medium containing either dodecane (C₁₂), tetradecane (C₁₄), hexadecane (C₁₆), octadecane (C₁₈), eicosane (C₂₀), docosane (C₂₂), tetracosane (C₂₄), hexacosane (C₂₆), or triacontane (C₃₀) as the sole carbon source. Samples were taken at the end of the exponential phase, and expression of the PalkB promoter was analyzed as described in the legend to Fig. 3. Lane M corresponds to the DNA size ladder. The two panels correspond to gels containing equivalent amounts of sample exposed for the same period of time.