TABLE 2.

Expression of the B. cepacia PalkB promoter in strain CPBC2 grown at the expense of different carbon sources

Carbon sourcea	PalkB promoter expression during:
	Exponential phase		Stationary phase
	Activityb	Repressionc	Activityb	Repressionc
TetOH	1,160	1	6,280	1
LB	74	NA	97	NA
LB+TetOH	100	12	99	63
Spent LB	74	15	115	54
Spent LB+TetOH	53	22	105	60
TetOH+citrate	82	14	102	61
TetOH+fumarate	64	18	80	78
TetOH+lactate	71	16	83	76
TetOH+pyruvate	101	11	97	65
TetOH+succinate	62	19	88	71
TetOH+glutamate	70	16	89	70
TetOH+acetate	129	9	100	62
TetOH+glycerol	140	8	80	78
TetOH+glucose	69	17	73	86
TetOH+fructose	79	15	123	51
TetOH+lactose	97	12	100	63
TetOH+arabinose	90	13	88	71
TetOH+myristic acid	292	4	379	16

^aCells were grown in fresh or spent LB medium in the absence or presence of tetradecanol (1% [wt/vol]) or in minimal salts medium in the presence of either tetradecanol (1% [wt/vol]) or a combination of tetradecanol and the indicated compound (30 mM for organic acids or sugars, 1% for myristic acid). TetOH, tetradecanol. 

^bActivity of the B. cepacia PalkB promoter (in Miller units) under each growth condition in exponentially growing or in stationary-phase cells. Values represent the averages of three independent assays; the standard deviation was less than 20%. 

^cRepression (in folds) indicates the decrease in promoter activity observed in each case relative to the activity observed when tetradecanol was the sole carbon source. NA, nonapplicable.