TABLE 2.
Effects of indole addition to conditioned medium from a tnaA mutant
| Strain | Growth conditiona | Mean β-galactosidase activity ± SD (Miller units)b |
|---|---|---|
| MT113 | LB | 299 ± 6 |
| CM (wild type) | 1,116 ± 57 | |
| CM (tnaA mutant) | 344 ± 17 | |
| CM (tnaA mutant) + 600 μM indole | 1,289 ± 39 | |
| CM (tnaA mutant) + 300 μM indole | 1,030 ± 79 | |
| CM (tnaA mutant) + 150 μM indole | 736 ± 11 | |
| MT114 | LB | 63 ± 2 |
| CM (wild type) | 252 ± 8 | |
| CM (tnaA mutant) | 210 ± 10 | |
| CM (tnaA mutant) + 600 μM indole | 320 ± 12 | |
| CM (tnaA mutant) + 300 μM indole | 289 ± 16 | |
| CM (tnaA mutant) + 150 μM indole | 244 ± 12 |
Conditioned medium (CM) was prepared at an A600 of = 1.4.
Cells were harvested at an A600 of 0.35.