Table 3.
Overview of primer/probe combinations used for RT-PCR.
| Gene name | Forward primer (5’-3’) | Reverse primer (5’-3’) | Probe |
|---|---|---|---|
| ACTB | GCCCAGCACGATGAAGAT | CGCCGATCCACACAGAGTA | AGGAGGAG |
| CRYAB | ATGGCAAACATGAAGAGCGC | GTAATGGTGAGAGGGTCCACA | TCTCCAGG |
| DNAJA4 | TTTCAGATCCAAAGAAAAGGGATATTT | ATGTCCATGGGTGAAGAGAAGCTG | GCTGCCTG |
| DNAJB1 | CATCGAAGTGAAGAAGGGGTG | AACAAAGACGATATCAGCTGGAA | GGAAGGAG |
| HSP90AA1 | CATGAAGACTCACAAAATCGGAAG | TTTCCTTCATTCTGGTGCAGT | CTGCCTCT |
| HSPA5 | TCCGGTCTACTATGAAGCCC | AATTCGAGTCGAGCCACCAA | CTTCCAGC |
| HSPB1 | CGGACGAGCTAACGGTTAAG | GTGAAGCACCGGGAGATGTAG | CTCCACCA |
| HSPD1 | GTTGGTCTTCAGGTTGTGGC | CTCCAAACACTGCACCACCA | CCTGGAGC |
| HSPH1 | CTCACAGTCTCCCCCTTCAC | GGCAGCTCGACATTCACCA | GGAGGCTG |
| IL-1α | AATAACCTGGAGGCCATCG | GCTAAAAGGTGCCGACCTG | CAGAGGAA |
| IL-6 | ACAAAAGTCCTGATCCAGTTCC | GTCATGTCCTGCAGCCACT | CAGCAGGC |
| IL-8 | TCTGTGTAAACATGACTTCCAAGC | CACTCCTTGGCAAAACTGC | CAGAGGAA |
| IL-12p40 | CCACATTCCTACTTCTCCCTGA | ACCGTGGCTGAGGTCTTGT | TCCAGGTC |
| P2RY6 | AGCTGTCTTTGCTGCCACA | GGGCTGAGGTCATAGCAAAC | GCTGGATG |
| TNF-α | AAGCCTGTAGCCCATGTTGT | GCTGGTTATCTGTCAGCTCCA | CCAGGAGG |