Table 4.
Plasma levels of cytokines according to the evaluated SNPs of the individuals included in the study.
| Cytokines | Gene SNP (rs) | Carrier | Mean (CI95%) | P-value | Mean (CI95%) | P-value | Mean (CI95%) | P-value | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| With TB | Without TB | With TB among HIV | Without TB among HIV | PTB | EPTB | ||||||
| IL-1β | CARD8 rs2043211 | A | 0.142 (0.061 - 0.222) | 0.202 (0.077 - 0.327) | 0.842 | 0.151 (0.048 - 0.255) | 0.129 (-0.063 - 0.321) | 0.997 | 0.069 (-0.022 - 0.159) | 0.319 (0.181 - 0.458) | 0.009 |
| CARD8 rs6509365 | A | 0.139 (0.061 - 0.217) | 0.165 (0.055 - 0.274) | 0.981 | 0.141 (0.039 - 0.242) | 0.102 (-0.046 - 0.251) | 0.975 | 0.065 (-0.021 - 0.151) | 0.301 (0.174 - 0.429) | 0.007 | |
| CASP-1 rs572687 | G | 0.055 (-0.039 - 0.149) | 0.056 (-0.104 - 0.215) | 1.000 | 0.039 (-0.082 - 0.159) | 0.038 (-0.189 - 0.264) | 1.000 | 0.025 (-0.114 - 0.163) | 0.048 (-0.078 - 0.173) | 0.994 | |
| IFI16 rs1101996 | C | 0.083 (0.013 - 0.154) | 0.081 (-0.032 - 0.194) | 1.000 | 0.058 (-0.03 - 0.147) | -0.009 (-0.165 - 0.147) | 0.864 | 0.06 (-0.025 - 0.145) | 0.087 (-0.026 - 0.2) | 0.977 | |
| IL-1β rs1143634 | G | 0.1 (0.009 - 0.19) | 0.042 (-0.125 - 0.208) | 0.931 | 0.1 (-0.019 - 0.218) | 0.058 (-0.235 - 0.352) | 0.994 | 0.054 (-0.054 - 0.163) | 0.176 (0.017 - 0.335) | 0.573 | |
| NLRP3 rs3806268 | G | 0.117 (0.044 - 0.191) | 0.132 (0.003 - 0.26) | 0.997 | 0.127 (0.039 - 0.214) | 0.153 (-0.021 - 0.326) | 0.993 | 0.052 (-0.039 - 0.143) | 0.165 (0.059 - 0.271) | 0.314 | |
| NLRP3 rs35829419 | C | 0.048 (-0.135 - 0.232) | 0.117 (-0.202 - 0.435) | 0.982 | 0.06 (-0.238 - 0.357) | 0.07 (-0.292 - 0.431) | 1.000 | 0.032 (-0.201 - 0.264) | 0.062 (-0.269 - 0.392) | 0.999 | |
| NLRP3 rs4612666 | C | 0.065 (-0.013 - 0.143) | 0.091 (-0.016 - 0.198) | 0.976 | 0.039 (-0.052 - 0.129) | 0.047 (-0.091 - 0.186) | 1.000 | 0.048 (-0.046 - 0.143) | 0.053 (-0.064 - 0.17) | 1.000 | |
| NLRP3 rs1539019 | C | 0.092 (0.015 - 0.17) | 0.068 (-0.062 - 0.197) | 0.985 | 0.093 (0.001 - 0.185) | 0.012 (-0.148 - 0.173) | 0.798 | 0.073 (-0.029 - 0.174) | 0.098 (-0.024 - 0.22) | 0.987 | |
| NLRP3 rs10754558 | C | 0.123 (0.054 - 0.193) | 0.042 (-0.088 - 0.172) | 0.652 | 0.109 (0.032 - 0.185) | 0.039 (-0.118 - 0.197) | 0.86 | 0.09 (-0.001 - 0.181) | 0.158 (0.057 - 0.259) | 0.684 | |
| IL-6 | CARD8 rs2043211 | A | 0.953 (0.793 - 1.114) | 0.353 (0.102 - 0.603) | <0.001 | 1.136 (0.966 - 1.307) | 0.342 (0.024 - 0.659) | <0.001 | 0.84 (0.646 - 1.035) | 1 (0.703 - 1.298) | 0.775 |
| CARD8 rs6509365 | A | 0.899 (0.744 - 1.053) | 0.385 (0.166 - 0.605) | 0.001 | 1.071 (0.905 - 1.237) | 0.45 (0.204 - 0.697) | <0.001 | 0.829 (0.641 - 1.018) | 0.827 (0.555 - 1.1) | 1.000 | |
| CASP-1 rs572687 | G | 0.946 (0.756 - 1.135) | 0.29 (-0.033 - 0.614) | 0.003 | 1.15 (0.948 - 1.352) | 0.289 (-0.094 - 0.671) | 0.001 | 0.615 (0.329 - 0.901) | 1.063 (0.803 - 1.322) | 0.074 | |
| IFI16 rs1101996 | C | 1.01 (0.869 - 1.15) | 0.357 (0.133 - 0.581) | <0.001 | 1.185 (1.035 - 1.334) | 0.354 (0.093 - 0.615) | <0.001 | 0.892 (0.715 - 1.069) | 1.023 (0.788 - 1.258) | 0.778 | |
| IL-1β rs1143634 | G | 1.003 (0.827 - 1.178) | 0.528 (0.197 - 0.858) | 0.067 | 1.202 (1.012 - 1.392) | 0.49 (-0.002 - 0.982) | 0.042 | 0.978 (0.757 - 1.199) | 0.867 (0.551 - 1.182) | 0.934 | |
| NLRP3 rs3806268 | G | 0.917 (0.774 - 1.061) | 0.319 (0.073 - 0.565) | <0.001 | 1.095 (0.954 - 1.236) | 0.391 (0.118 - 0.663) | <0.001 | 0.754 (0.571 - 0.938) | 0.932 (0.72 - 1.145) | 0.516 | |
| NLRP3 rs35829419 | C | 0.726 (0.36 - 1.091) | 0.155 (-0.478 - 0.789) | 0.406 | 0.931 (0.434 - 1.429) | 0.339 (-0.264 - 0.941) | 0.432 | 0.578 (0.093 - 1.064) | 0.857 (0.169 - 1.546) | 0.916 | |
| NLRP3 rs4612666 | C | 1.01 (0.855 - 1.166) | 0.282 (0.07 - 0.494) | <0.001 | 1.226 (1.073 - 1.378) | 0.386 (0.155 - 0.617) | <0.001 | 0.985 (0.789 - 1.182) | 0.83 (0.591 - 1.069) | 0.703 | |
| NLRP3 rs1539019 | C | 0.913 (0.759 - 1.066) | 0.317 (0.067 - 0.568) | <0.001 | 1.153 (1 - 1.307) | 0.383 (0.12 - 0.646) | <0.001 | 0.838 (0.63 - 1.047) | 0.86 (0.609 - 1.11) | 0.999 | |
| NLRP3 rs10754558 | C | 1.075 (0.939 - 1.211) | 0.231 (-0.017 - 0.479) | <0.001 | 1.279 (1.156 - 1.402) | 0.316 (0.072 - 0.56) | <0.001 | 1.03 (0.847 - 1.214) | 0.987 (0.781 - 1.192) | 0.985 | |
| IL-18 | CARD8 rs2043211 | A | 1.363 (1.201 - 1.524) | 1.333 (1.07 - 1.595) | 0.997 | 1.591 (1.395 - 1.788) | 1.684 (1.293 - 2.076) | 0.975 | 1.329 (1.149 - 1.509) | 1.588 (1.313 - 1.864) | 0.345 |
| CARD8 rs6509365 | A | 1.31 (1.155 - 1.465) | 1.417 (1.191 - 1.643) | 0.857 | 1.516 (1.33 - 1.703) | 1.773 (1.483 - 2.062) | 0.467 | 1.28 (1.109 - 1.45) | 1.523 (1.277 - 1.77) | 0.303 | |
| CASP-1 rs572687 | G | 1.333 (1.146 - 1.519) | 1.43 (1.112 - 1.748) | 0.947 | 1.69 (1.463 - 1.917) | 1.667 (1.241 - 2.094) | 1.000 | 1.303 (1.036 - 1.571) | 1.458 (1.215 - 1.701) | 0.801 | |
| IFI16 rs1101996 | C | 1.361 (1.224 - 1.498) | 1.413 (1.185 - 1.641) | 0.976 | 1.695 (1.53 - 1.86) | 1.636 (1.332 - 1.941) | 0.986 | 1.418 (1.259 - 1.577) | 1.381 (1.17 - 1.593) | 0.991 | |
| IL-1β rs1143634 | G | 1.548 (1.379 - 1.718) | 1.354 (1.034 - 1.673) | 0.716 | 1.826 (1.615 - 2.036) | 1.668 (1.125 - 2.211) | 0.95 | 1.617 (1.423 - 1.811) | 1.522 (1.244 - 1.799) | 0.939 | |
| NLRP3 rs3806268 | G | 1.353 (1.21 - 1.496) | 1.336 (1.072 - 1.6) | 0.999 | 1.684 (1.52 - 1.847) | 1.72 (1.365 - 2.076) | 0.998 | 1.36 (1.188 - 1.533) | 1.466 (1.266 - 1.665) | 0.82 | |
| NLRP3 rs35829419 | C | 1.301 (0.939 - 1.664) | 1.37 (0.742 - 1.999) | 0.998 | 1.338 (0.787 - 1.889) | 1.77 (1.1 - 2.439) | 0.751 | 1.497 (1.059 - 1.936) | 1.014 (0.391 - 1.636) | 0.6 | |
| NLRP3 rs4612666 | C | 1.424 (1.273 - 1.576) | 1.458 (1.243 - 1.674) | 0.993 | 1.734 (1.565 - 1.903) | 1.768 (1.493 - 2.042) | 0.997 | 1.468 (1.289 - 1.648) | 1.44 (1.222 - 1.657) | 0.996 | |
| NLRP3 rs1539019 | C | 1.345 (1.193 - 1.498) | 1.39 (1.126 - 1.654) | 0.989 | 1.611 (1.441 - 1.782) | 1.725 (1.415 - 2.035) | 0.91 | 1.345 (1.155 - 1.536) | 1.459 (1.23 - 1.688) | 0.846 | |
| NLRP3 rs10754558 | C | 1.43 (1.294 - 1.565) | 1.606 (1.341 - 1.871) | 0.602 | 1.718 (1.577 - 1.859) | 1.949 (1.642 - 2.257) | 0.531 | 1.478 (1.308 - 1.648) | 1.471 (1.281 - 1.662) | 1.000 | |
| IL-33 | CARD8 rs2043211 | A | 1.346 (1.142 - 1.55) | 1.076 (0.757 - 1.394) | 0.476 | 1.419 (1.172 - 1.665) | 0.706 (0.248 - 1.164) | 0.038 | 1.13 (0.896 - 1.365) | 1.605 (1.246 - 1.964) | 0.094 |
| CARD8 rs6509365 | A | 1.38 (1.184 - 1.577) | 1.024 (0.745 - 1.303) | 0.146 | 1.447 (1.208 - 1.685) | 0.796 (0.441 - 1.151) | 0.018 | 1.167 (0.941 - 1.393) | 1.592 (1.265 - 1.919) | 0.105 | |
| CASP-1 rs572687 | G | 1.616 (1.372 - 1.861) | 1.194 (0.777 - 1.61) | 0.275 | 1.635 (1.346 - 1.925) | 0.89 (0.344 - 1.437) | 0.07 | 1.431 (1.057 - 1.805) | 1.606 (1.267 - 1.946) | 0.883 | |
| IFI16 rs1101996 | C | 1.585 (1.406 - 1.764) | 1.075 (0.789 - 1.361) | 0.008 | 1.628 (1.415 - 1.84) | 0.806 (0.436 - 1.176) | 0.001 | 1.517 (1.294 - 1.74) | 1.513 (1.217 - 1.808) | 1.000 | |
| IL-1β rs1143634 | G | 1.501 (1.274 - 1.728) | 1.266 (0.838 - 1.694) | 0.778 | 1.574 (1.302 - 1.847) | 0.85 (0.143 - 1.556) | 0.234 | 1.36 (1.08 - 1.641) | 1.6 (1.199 - 2.001) | 0.748 | |
| NLRP3 rs3806268 | G | 1.544 (1.357 - 1.731) | 1.035 (0.714 - 1.356) | 0.03 | 1.608 (1.399 - 1.816) | 0.863 (0.46 - 1.265) | 0.008 | 1.35 (1.111 - 1.588) | 1.583 (1.306 - 1.859) | 0.51 | |
| NLRP3 rs35829419 | C | 1.472 (0.995 - 1.95) | 1.257 (0.429 - 2.085) | 0.969 | 1.56 (0.844 - 2.276) | 1.274 (0.406 - 2.141) | 0.957 | 1.41 (0.786 - 2.034) | 1.517 (0.632 - 2.403) | 0.997 | |
| NLRP3 rs4612666 | C | 1.514 (1.313 - 1.715) | 1.097 (0.824 - 1.371) | 0.051 | 1.576 (1.361 - 1.792) | 0.969 (0.642 - 1.295) | 0.013 | 1.393 (1.14 - 1.647) | 1.475 (1.167 - 1.783) | 0.971 | |
| NLRP3 rs1539019 | C | 1.572 (1.372 - 1.772) | 1.057 (0.731 - 1.383) | 0.022 | 1.673 (1.454 - 1.892) | 0.893 (0.517 - 1.268) | 0.002 | 1.457 (1.19 - 1.724) | 1.555 (1.235 - 1.875) | 0.958 | |
| NLRP3 rs10754558 | C | 1.662 (1.488 - 1.837) | 0.96 (0.641 - 1.279) | <0.001 | 1.709 (1.528 - 1.889) | 0.858 (0.5 - 1.216) | <0.001 | 1.602 (1.37 - 1.835) | 1.572 (1.312 - 1.832) | 0.997 | |
P-values were calculated using the unconditional logistic regression model. Associations were considered significant with a value of P < 0.05. TB, tuberculosis; aOR, adjusted odds ratio; 95% CI, 95% confidence interval; PTB, Pulmonary TB; EPTB, Extrapulmonary TB. The AIM2 rs2276405 polymorphisms had insufficient observations and/or no way to calculate the standard error (all observations from one or more groups were equal to the lower detection limit of the assay) for the analyses between the groups with vs. without TB, with TB vs. without TB among HIV and PTB vs. EPTB. A, A allele; C, C allele; G, G allele. Bold indicate statistically significant results.