Figure 6.

Invasion phenotypes of P.falciparum clinical isolates before and after short-term cryopreservation. The assays were set up between 24- and 36-hours following sample processing and the parasites were incubated for another 18 to 24 hours prior to flow cytometric analysis. For each isolate, the invasion phenotype of freshly culture adapted parasites (black bars) was compared to that obtained after three months (dark grey bars), six months (light grey bars) and twelve months (white bars) post cryopreservation. Kruskal Wallis was conducted to test for statistical differences in invasion efficiency of fresh versus cryopreserved isolates. Red stars denote significant differences associated with changes in invasion profile of a given treatment and black stars depict significant differences with no changes in the invasion profile.