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. 2022 Sep 20;13:998756. doi: 10.3389/fimmu.2022.998756

Figure 1.

Figure 1

Malaria parasite infections in mice alter host spleen mRNA m6A methylation levels. (A) Schematic of the experimental design and MeRIP-seq protocol used in this study to identify differential m6A methylation profiles in mice spleens following infection with Plasmodium yoelii parasites. (B) LC-MS/MS quantification of m6A level in poly(A)-selected and RiboMinus-treated mRNAs isolated from the spleens of noninfected, P. y. nigeriensis N67-, P. y. nigeriensis N67C-, P. y. yoelii YM-, or P. y. yoelii 17XNL- infected mice. (C) LC-MS/MS quantification of m6A level in poly(A)-selected and RiboMinus-treated mRNAs isolated from N67, N67C, YM and 17XNL parasites. Values are the means  ± SD, n = 3. *P < 0.05; **P < 0.01; ***P < 0.001, Student’s t test.