Figure 3. The deficiency of slco2b1 induces a decrease of HSPC in the CHT.

• A
  WISH for cmyb expression at 60 hpf in wild‐type and slco2b1 ^−/− embryos.
• B
  Quantification of cmyb‐expressing cells. Each n represents the number of cmyb‐expressing cells for each embryo (biological replicates). Each experiment has been repeated three independent times.
• C
  Schematic indicating the imaging area in the tail at 60 hpf, as indicated by the black box; fluorescence imaging in the CHT of kdrl:mCherry;cmyb:GFP embryos injected with control‐ and slco2b1‐Mos.
• D
  Quantification of HSPCs associated with ECs. Each n represents the number of yellow spots for each embryo (biological replicates). Each experiment has been repeated three independent times.
• E
  Experimental outline and quantification of time‐lapse live imaging in controls and slco2b1‐morphants cmyb:GFP ⁺ cells, using Imaris software.

Data information: Center values denote the mean, and error values denote s.e.m. The statistical analysis was completed using an unpaired two‐tailed t‐test. ***P < 0.001. Scale bar is 500 μm (A); 200 μm (C).

Source data are available online for this figure.