Figure 5. Slco2b1 is necessary to import PGH2 into ECs.

• A
  Fluorescence imaging of the CHT of double transgenic cd45:CFP‐NTR/runx1:mcherry embryos injected with control‐ or slco2b1‐morpholinos, after treatment with DMSO.
• B
  Fluorescence imaging of the CHT of double transgenic cd45:CFP‐NTR/runx1:mcherry embryos injected with control‐ or slco2b1‐morpholinos, after treatment with DMSO+MTZ_.
• C
  Fluorescence imaging of the CHT of double transgenic cd45:CFP‐NTR/runx1:mcherry embryos injected with control‐ or slco2b1‐morpholinos, after treatment with DMSO+MTZ + PGH2.
• D
  Quantification of runx1:mcherry‐positive cells. Each dot represents the number of red cells for each embryo (biological replicates). Each experiment has been repeated three independent times.
• E
  Quantification of cd45:CFP‐positive cells_. Each dot represents the number of blue cells for each embryo (biological replicates). Each experiment has been repeated three independent times. Data information for (D) and (E): Statistical analysis was completed using one‐way ANOVA and multiple comparison tests. ****P < 0.0001. Center values denote the mean, and error bars denote s.e.m.

Scale bar is 200 μm (A–C).

Source data are available online for this figure.