Figure 8. Myeloid and endothelial cells are very close to HSPCs in the mouse fetal liver.

• A
  Immunohistochemistry on paraffin sections for cKit (HSPCs marker) and CD31 (endothelial cell marker) at E13 and E16 stages of mouse fetal liver.
• B
  Distance distribution between cKit^high cells and endothelial cells (n = 79 from three E13 fetal livers; n = 92 from three E16 fetal livers), binned into 20 μm intervals. Statistical analysis was completed using a t‐test. *P < 0.01; **P < 0.001; ***P < 0.0001. Center values denote the mean, and error values denote s.e.m.
• C
  Immunohistochemistry on paraffin sections for cKit (HSPCs marker) and F4/80 (macrophage cell marker) at E13 and E16 stages of mouse fetal liver.
• D
  Distance distribution between cKit^high cells and macrophages (n = 54 from three E13 fetal livers; n = 64 from three E16 fetal livers), binned into 5 μm intervals. Statistical analysis was completed using a t‐test.
• E
  Immunohistochemistry on paraffin sections for cKit (HSPCs marker) and MBP (neutrophil cell marker) at E13 and E16 stages of mouse fetal liver.
• F
  Distance distribution between cKit^high cells and neutrophils (n = 45 from three E13 fetal livers; n = 53 from three E16 fetal livers), binned into 20 μm intervals. Statistical analysis was completed using a t‐test ***P < 0.001.

Data information: All nuclei were marked with DAPI. Scale bar is 20 μm (A–C–E).