Figure 3. A mouse model for the simultaneous measurement of energy metabolism in defined immune cell populations.

Perceval^fl/fl mice were crossed with Vav‐iCre animals to achieve expression of the metabolic reporter in all cells of hematopoietic origin.

• A
  Gating strategy used from lymph node cells.
• B
  Reporter expression (as measured by the ATP signal) is detected in all immune cell populations analyzed. Negative dotted lines correspond to the value measured in the C57Bl/6 wild‐type mouse.
• C
  ATP:ADP ratios measured at steady state in B cells, T cells, NKs, DCs, monocytes, and neutrophils.
• D, E
  SPICE‐Met was performed on various lymph node immune cell types simultaneously. (D) ATP:ADP ratios were normalized to the untreated condition and shown for different populations upon treatment with DMSO+H₂O as control, oligo, 2‐DG, or oligo+2‐DG. (E) OXPHOS and glucose dependencies were calculated for B cells, T cells, NKs, DCs, monocytes, and neutrophils.

Data information: Results are pooled from three independent experiments (n = 6). Data are represented as mean ± SEM.