Fig. 3.

Validation of TfR hAPI KI mice. A Top: TfR protein levels in brain lysates of wild-type (WT), heterozygous (Het) and hAPI knock-in (KI) mice. Actin is used as a loading control. Uncropped western blot can be found in Additional file 2: Fig. S2. Bottom: Quantification of TfR protein levels normalized to WT levels. Bar graphs represent mean ± SEM (n = 3 for KI; n = 4 for WT and Het). B C57BL/6 WT and TfR hAPI KI mice body temperature measurements after 250 nmol/kg intravenous injections of Nb62 fused to NT(8-13). Bar graphs represent mean ± SEM (n = 3 per group). Statistical test: two-way ANOVA with repeated measures and Sidak’s multiple comparisons test. (significant time*genotype interaction effect ****p < 0.0001)