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Desulfo-CoA inhibition of phosphotransacetylase. Desulfo-CoA inhibition was determined by the coupled assay in the presence of various levels of CoA and a fixed level of acetyl phosphate. Data were plotted as 1/v versus 1/[CoA]. Ki was determined using the equation for a competitive inhibitor, v = Vmax{Km/[CoA](1 + [I]/Ki) + 1}. The symbols represent no added desulfo-CoA (●), 10 μM desulfo-CoA (■), and 25 μM desulfo-CoA (▴).
