TABLE 1.
Kinetic properties of the wild type and Arg 87 and Arg 133 variants
| Enzyme | CoA
|
dCoA
|
[kcat/Km](CoA) [kcat/Km](dCoA) | ||||
|---|---|---|---|---|---|---|---|
| kcat-(s−1)a | Km (mM)a | kcat/Km (mM−1s−1) | kcat (s−1)a | Km (mM)a | kcat/Km (mM−1s−1) | ||
| Wild type | 4,880 ± 448 | 0.07 ± 0.01 | 70,000 | 272 ± 14 | 1.4 ± 0.1 | 198 | 350 |
| R87A | 1,225 ± 109 | 0.9 ± 0.2 | 1,400 | 184 ± 38 | 6 ± 2 | 31 | 45 |
| R87Q | 1,390 ± 64 | 1.1 ± 0.1 | 1,250 | 98 ± 23 | 1.3 ± 0.7 | 76 | 17 |
| R87K | 373 ± 25 | 0.57 ± 0.09 | 650 | 41 ± 7 | 3.9 ± 0.9 | 10 | 65 |
| R87E | 17 ± 1 | 0.27 ± 0.06 | 63 | 23 ± 2 | 0.8 ± 0.1 | 30 | 2 |
| R133A | 631 ± 28 | 0.36 ± 0.05 | 1,700 | 0.75 ± 0.08 | 1.3 ± 0.4 | 0.6 | 2,800 |
| R133Q | 1,146 ± 55 | 0.7 ± 0.1 | 1,700 | 28 ± 4 | 4 ± 1 | 7 | 250 |
| R133K | 418 ± 36 | 0.11 ± 0.03 | 3,800 | 8 ± 0.4 | 0.8 ± 0.1 | 10 | 380 |
| R133E | 44 ± 5 | 0.7 ± 0.1 | 60 | 0.28 ± 0.02 | 1 ± 0.2 | 0.3 | 200 |
a
Values are given as means ± standard deviations.