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. 2022 Oct 4;13:494. doi: 10.1186/s13287-022-03049-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Identification of ADSCs and ADSCs-derived exosomes. A Cytomorphology of P3-ADSCs was observed under optic microscope. B Flow cytometry analysis for ADSCs markers (including CD44, CD73, CD90, CD105) in P3-ADSCs. C Transmission electron microscopic (TEM) analysis for ADSCs-derived exosomes (scale bar 100 nm). D Western blot analysis of exosomal protein markers Alix, Tsg101, CD63 and ER marker Calnexin in ADSC-EXO lysate, ADSC protein as control. E–G Nanoparticle tracking analysis (NTA) for ADSC-EXO size distribution, concentration and intensity