Fig. 7.

Nuclear translocation of CREB3L1 by KPNA2 activates IL-1α expression. A The cytokines changed in the supernatant of 8505C cells (left panel) and co-culture supernatant of 8505C-CAFs (right panel) after CREB3L1 knockdown were detected by human cytokine array. B-C Validation of IL-1α in the supernatant by ELISA kit. D The proportion of α-SMA⁺ CAFs was analyzed by flow cytometry. CAFs were co-cultured with 8505C cells and were exposed to 1 ng/mL IL-1α for 48 h. *P < 0.05, **P < 0.01 NC versus CREB3L1-KD or CAF + NC versus CAF + CREB3L1-KD. A nucleocytoplasmic separation assay (E) and immunofluorescence (F) were used to detect the localization of CREB3L1 in PTC and ATC cell lines. (G) The expressions of the nuclear transport receptor karyopherin family members were analyzed in four integrated datasets. H Co-immunoprecipitation was used to verify the interaction between CREB3L1 and KPNA2 in different thyroid cancer cells. I WB was used to detect the cytoplasmic and nuclear expression of CREB3L1 after KPNA2 silencing in 8505C cells