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. Author manuscript; available in PMC: 2023 Jun 21.
Published in final edited form as: Annu Rev Biochem. 2022 Mar 23;91:89–106. doi: 10.1146/annurev-biochem-032620-105728

Table 1.

Interenzyme and enzyme–subcellular structure interactions involving DNPB enzymes in human cell lines detected by various techniques. Evidence for interactions detected in transiently overexpressed enzymes

DNPB enzyme interactions probed Technique
Fluorescence imaging of tagged enzymes Coimmunoprecipitation Luminescence-based mammalian interactome (LUMIER) assay Bimolecular fluorescence complementation (BiFC) assay
Enzyme participants PPAT, GART, PFAS, PAICS, ADSL, ATIC, IMPDH, ADSS (15, 16, 34, 64) PFAS, PAICS, ATIC (18)
PFAS–PAICS, MTHFD1, GART, IMPDH2 (65)		 ND GART, PFAS, PAICS, ADSL, ATIC (35)
Mitochondria and microtubule Mitochondria–PFAS (17, 23, 28)
Microtubule–PFAS (23,37)		 Mitochondria–PFAS (28) ND ND
Chaperone machinery HSP90/HSP70/DNAJ-C7/DNAJ A1–PFAS/PPAT/PAICS (18) HSP90/HSP70/DNAJ-C7–PFAS/PPAT (18, 56, 65) HSP90–PPAT/PFAS (56) ND

Abbreviations: ADSL, adenylosuccinate lyase; ADSS, adenylosuccinate synthetase; ATIC, 5-aminoimidazole-4-carboxamide nucleotide formyltransferase/IMP cyclohydroxylase; DNPB, de novo purine biosynthesis; GART, phosphoribosylglycinamide formyltransferase; HSP, heat shock protein; IMPDH, inosine monophosphate dehydrogenase; MTHFD, methylenetetrahydrofolate dehydrogenase/cyclohydrolase (cytosolic isoform); ND, not determined; PAICS, phosphoribosyl aminoimidazole succinocarboxamide synthase; PFAS, phosphoribosyl formylglycinimidine transferase; PPAT, phosphoribosyl pyrophosphate amidotransferase.