TABLE 3.
NADP and NAD concentrations and their redox state in cellular extractsa
| Strain | Treatment | NADPtotal (μmol of NADP/mg of Chlb) | % NADP reduced | NADtotal (μmol of NAD/mg of Chl) | % NAD reduced |
|---|---|---|---|---|---|
| WT | Air | 6.4 ± 0.1 (5.0 ± 0.3) | 75 ± 10 (70 ± 5) | 0.6 ± 0.2 (0.9 ± 0.1) | 65 ± 15 (55 ± 6) |
| SDH Defd | Air | 2.5 ± 0.1 (2.1 ± 0.4) | 50 ± 15 (55 ± 6) | 0.1 ± 0.3 (0.4 ± 0.1) | 50 ± 21 (50 ± 5) |
| NDH-2 Def | Air | 8.7 ± 0.1 (6.6 ± 0.1) | 35 ± 20 (25 ± 6) | 0.8 ± 0.2 (0.6 ± 0.1) | 100 ± 5 (100 ± 3) |
| WTc | Air + 3% CO2 | 2.9 ± 0.1 (4.0 ± 0.4) | 50 ± 10 (50 ± 10) | 0.3 ± 0.4 (0.3 ± 0.1) | 70 ± 20 (45 ± 5) |
| NDH-1 Def | Air + 3% CO2 | 0.5 ± 0.1 (0.8 ± 0.1) | 100 ± 4 (100 ± 3) | 0.02 ± 0.3 (0.1 ± 0.1) | 70 ± 20 (39 ± 9) |
a
The values reported are averages of three separate determinations using cells grown at 45 μmol of photons m−2 s−1. The standard deviations are indicated. Concentrations were determined by NAD and NADP cycling reactions and fluorescence-based HPLC (values in parentheses) as described in the text.
b
Chl, chlorophyll.
c
WT, wild type.
d
Def, deficient.