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. 2022 Sep 20;13:1003986. doi: 10.3389/fimmu.2022.1003986

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Copyright © 2022 Pernold, Lagumdzic, Stadler, Mair, Jäckel, Schmitt, Ladinig, Knecht, Dürlinger, Kreutzmann, Martin, Sawyer and Saalmüller

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Total numbers of CD4⁺ T cells and the development of T_CM and T_EM CD4⁺ cells over a study period of 126 weeks. (A) Gating strategy to distinguish CD4 T cells and CD8α/CD27-defined subpopulations on the basis of a representative EGM (male, 18 weeks of age). (B) Shows the total CD4⁺ T cell counts calculated as described above. Sexes are represented by black (male) and red colors (female). (C, D) Changes in the composition of the CD4/CD8α/CD27-defined T cell subsets representing T_Naive (CD4⁺CD8α^-CD27⁺), activated and T_CM (CD4⁺CD8α⁺CD27⁺) and T_EM (CD4⁺CD8α⁺CD27^-) in males (C) and females (D). + marks the first bleeding time point after transfer and vaccination, ++ marks the first bleeding time point after booster vaccinations.