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. 2022 Sep 20;13:1003986. doi: 10.3389/fimmu.2022.1003986

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Copyright © 2022 Pernold, Lagumdzic, Stadler, Mair, Jäckel, Schmitt, Ladinig, Knecht, Dürlinger, Kreutzmann, Martin, Sawyer and Saalmüller

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Proliferation assay of CellTrace™Violet-stained PBMCs after restimulation with baculovirus-expressed PCV2-ORF2 protein. PBMCs cultivated in medium or stimulated with an irrelevant baculovirus-expressed protein (GP64) were used as negative controls, ConA-stimulated PBMCs served as positive control. (A) Shows the percentage of reacting T cells within live lymphocytes of three representative EGMs. (B) shows the percentage of proliferating CD4⁺ T cells within live lymphocytes of three EGMs. (C) Shows the reactivity of the CD8α/CD27-defined CD4⁺ T- cell subpopulation of three EGMs. Naive CD4⁺ T cells (T_Naive), central memory CD4⁺ T cells (T_CM) and effector memory CD4⁺ T cells (T_EM).