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. 2022 Sep 20;13:1003986. doi: 10.3389/fimmu.2022.1003986

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Copyright © 2022 Pernold, Lagumdzic, Stadler, Mair, Jäckel, Schmitt, Ladinig, Knecht, Dürlinger, Kreutzmann, Martin, Sawyer and Saalmüller

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intracellular cytokine staining for the detection of antigen-specific TNF-α and IFN-γ producing T cells after restimulation with PCV2-ORF2 protein. (A-D) Distribution of TNF-α and IFN-γ within T_Naive, T_CM and T_EM CD4⁺ cells after 18h of stimulation summarized for six different EGMs.