TABLE 1.

Purification of aminoacylase from P. furiosus

Step	Activitya (U)	Protein (mg)	Sp act (U/mg)	Purification (fold)	Recovery (%)
Cell extract	8,800	26,000	0.3	1	100
QFF Sepharose	5,800	1,600	3.7	11	65
Phenyl Sepharose	4,100	160	25.4	75	46
QHP Sepharose	1,050	27	39.0	115	12
Hydroxyapatite	700	7	100	290	8
HiTrap Q	660	3	200	580	7

^aActivity was measured at 100°C by using N-acetyl-l-methionine (30 mM) as the substrate.