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Kinetic parameters for substrates of P. furiosus aminoacylase
| Substratea | Km (mM) | Vmax (μmol/ min/mg) | kcatb (s−1) | kcat/km (mM−1s−1) |
|---|---|---|---|---|
| N-Acetyl-l-methionine | 6.6 | 500 | 2,720 | 412 |
| N-Formyl-l-methionine | 13.0 | 99 | 538 | 41 |
| N-Chloroacetyl-l-valine | 2.5 | 500 | 2,720 | 1,090 |
All assays were carried out at 100°C in 50 mM Bis-Tris HCl, pH 6.5.
Based on a minimum molecular mass of 42.06 kDa.
