Alignments of the amino acid sequences of the
α-subunit (CaaD1) and β-subunit (CaaD2) of CaaD with the seven 4-OT
sequences (A and B) and with the amino-terminal sequences of the
subunits of the CaaD isolated from strain FG41 (C). Residues conserved
throughout all sequences are indicated by an asterisk. Dashes represent
residues absent in other sequences. The catalytically important
residues in 4-OT are marked with + and shown in boldface. The region of
highest sequence identity among CaaD1 and the 4-OT sequences is boxed.
Ppa, P. pavonaceae; Bs, B. subtilis; Psp,
Pseudomonas sp.; Pp, P. putida; Ps, P.
stutzeri; Sa, S. aromaticivorans; Csp, coryneform
bacterial strain.