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Activities of wild-type and mutant CaaD for trans-3-chloroacrylic acid (CAA) and trans-3-bromoacrylic acid (BAA)
| Enzyme | Dehalogenase
sp acta (mU/mg of protein)
|
|
|---|---|---|
| CAA | BAA | |
| Wild type | 1,500 | 3,250 |
| αP1A | 1,260 | 3,050 |
| βP1A | <5 | 15 |
| αR11A | <5 | <5 |
| αR11K | 110 | 240 |
| αF39A | 350 | 480 |
| αF39Y | 240 | 300 |
Activities were measured in cell extracts containing similar amounts of dehalogenase. Halide production with 5 mM substrate was determined at 30°C and pH 8.2.
