| SAS-6 |
3xFlag N-terminal CRISPR tag (this study). |
Single copy C-terminal GFP tag expressed under the endogenous promoter without the endogenous locus [57]. |
AB raised in rabbit against full-length protein [43]. |
| SAS-5 |
N-terminal AB raised in rabbit against aa 1–122 [58]. |
|
|
| SAS-1 |
3xFlag N-terminal CRISPR tag (this study). |
3xFlag C-terminal CRISPR tag (this study). |
|
| SAS-4 |
AB raised in rabbit against aa 350–517 [19]. |
GFP C-terminal CRISPR tag [59]. |
|
| α-tubulin |
N-terminal AB raised in rabbit against the first 100 aa (EP1332Y, Abcam, ab52866). |
C-terminal antibody raised in rat against the C-terminal Tyrosine α-tubulin (EMD Millipore, MAB1864). |
|
| HYLS-1 |
Single copy N-terminal mCherry tagged transgene expressed under the endogenous promoter in presence of endogenous HYLS-1 [60]. |
|
|
| PCMD-1 |
Single copy N-terminal GFP tag expressed without the endogenous locus [21]. |
Single copy C-terminal GFP tag expressed without the endogenous locus [21]. |
|
| SPD-5 |
AB raised in rabbit against the C-terminal 18 aa [24]. |
|
|
| SPD-2 |
GFP C-terminal CRISPR tag [61]. |
tagRFP N-terminal CRISPR tag [62]. |
|
| SAS-7 |
tagRFP N-terminal CRISPR tag [62]. |
|
|
| TBG-1 |
AB raised in rabbit against the C-terminal 17 aa [28]. |
|
|
| MZT-1 |
GFP N-terminal CRISPR tag [27]. |
|
|
