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. 2022 Sep 22;18(9):e1010837. doi: 10.1371/journal.ppat.1010837

Fig 5. Pirk-like interferes with amyloidal aggregates formed by PGRP-LC, PGRP-LE, and IMD.

Fig 5

(A) Amyloidal aggregate staining of Aag2 cells transiently transfected with V5-tagged PGRP-LC, PGRP-LE or IMD. Scale bar: 10 μm. (B) GST pull-down assay between PGRP-LC, PGRP-LE, IMD, and Pirk-like. The interactions of His-Pirk-like with GST-PGRP-LC, GST-PGRP-LE or GST-IMD were observed in the pull-down assay and confirmed using western blots with anti-GST and anti-His antibodies. (C-E) ThT fluorescence in Aag2 cells co-expressed with Ha-tagged PGRP-LC (C), PGRP-LE (D) or IMD (E), and either an empty vector (mock) or a V5-Pirk-like. Scale bar: 10 μm. (F) Expression of Rel2, Dpt, DefC and CecB in iEGFP and iEcR mosquitoes infected with E. cloacae in the presence of ThT. Acetic acid was used as control. Data are shown as mean ± SEM. ****p < 0.0001; ns, not significant (one-way ANOVA followed by Bartlett’s test).