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. 2022 Oct 4;17(10):e0272097. doi: 10.1371/journal.pone.0272097

Fig 1. PKM2 is efficiently deleted in mature mSC of mutant mice.

Fig 1

A- Quantitative RT-PCR on sciatic nerve extracts shows decreased PKM1 and increased PKM2 expressions during mSC maturation. Normalized on P2 values. n = 4 animals. B- Immunostaining on mouse sciatic nerve sections at increasing ages (a, b: 4 days postnatal (d); c, d: 15 days postnatal; e, f: 30 days postnatal; g, h: 5 months postnatal (m)) shows decreased PKM1 (left panels) and increased PKM2 (right panels) expressions in mSC over time. m: months. Sc: Schwann cell; a: axon. C- Western blots on sciatic nerve extracts show lower PKM2 and higher PKM1 amounts in Mutant versus Control mice sciatic nerves (2 months old). Actin serves as loading control. n = 3 mice. D- Quantitative RT-PCR on sciatic nerve extracts shows decreased PKM2 and increased PKM1 expressions in Mutant (Mut, n = 7) versus Control (Cont, n = 5) mouse sciatic nerves (samples from 3 and 12 months old mice were pooled). E- Immunostainings on sciatic nerve sections show PKM2 lower in Mutant mSC than in Control mice (panel j vs panel l) while PKM1 is higher (panel i vs panel k)(2 months old). F- The ratio of Laconic probe was measured in mSC of Mutant (n = 11 mice, 41 cells) and Control (n = 11 mice, 45 cells) anesthetized mice and normalized to the mean of Control values (7 to 10 weeks old mice). G-Biochemical measure of lactate shows a reduced concentration in Mutant versus Control sciatic nerves (n = 6 mice)(samples from 4 and 12 months old mice were pooled). All scale bars = 10μm. Two-tailed Student t-test. Error bars represent SD (A, D) or SEM (F). AU: arbitrary unit.