Table 2.
Description of the Four Most Common Histology and Immunohistochemistry Stains Used on Specimens Collected from the Porcine SCI Model1,26,44
| Methodologies | N | Function |
|---|---|---|
| Histology | ||
| H&E Eriochrome cyanine R Luxol fast blue Azur-eosin |
17 16 7 2 |
Distinguish nuclear and cytoplasmic parts Stain myelinated fibers Stain myelinated fibers Distinguish spared from injured tissue |
| Immunohistochemistry | ||
| GFAP NF Iba1 NeuN |
10 9 5 4 |
Expressed by astrocytes Found in cytoplasm of neurons Expressed in microglia Neuronal nuclear antigen |
GFAP, glial fibrillary acidic protein; H&E, hematoxylin and eosin; NF, neurofilaments; Iba1, ionized calcium-binding adaptor molecule 1; NeuN, neuronal nuclear protein.