FIG. 2.

HPLC analysis of the enzymatically formed benzylsuccinyl-CoA isomer. Chemically synthesized (solid line) and enzymatically synthesized (broken line) benzylsuccinyl-CoA were separated by HPLC and monitored by their absorption at 260 nm. The dotted line represents the hypothetical elution profile of 3-benzylsuccinyl-CoA; it represented 30 to 40% of the amount of chemically synthesized benzylsuccinyl-CoA. The curve was calculated by subtracting the elution profile of enzymatically synthesized benzylsuccinyl-CoA from that of the chemically synthesized isomer mixture. The structures of the benzylsuccinyl-CoA isomers were inferred from correlation with NMR data as shown.