TABLE 1.
Purification of succinyl-CoA:benzylsuccinate CoA-transferase from T. aromaticaa
| Purification step | Volume (ml) | Protein (mg) | Activity (nmol min−1) | Specific activity (nmol min−1 mg−1) | Enrichment factor | Yield (%) |
|---|---|---|---|---|---|---|
| Crude extract | 12.5 | 56.3 | 13.3 | 23.7 | 1 | 100 |
| DEAE-Sepharose | 60 | 17.7 | 12.1 | 68.1 | 2.9 | 91 |
| Hydroxyapatite | 60 | 84 | 7.5 | 89.4 | 3.8 | 73 |
| Q-Sepharose | 70 | 22.5 | 2.3 | 100 | 4.2 | 45 |
| Gel filtration | 8 | 0.5 | 0.14 | 300 | 12.7 | 9 |
a
Yield values have been corrected for the fractions of active pools which were not used for the next purification steps.